vent cap cell culture flasks (Corning Life Sciences)

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Corning Life Sciences vent cap cell culture flasks
Vent Cap Cell Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vent+cap+cell+culture+flasks/cell+culture+plates+with+vent+caps/pmc11385518__mmc2-193-25-30
Average 90 stars, based on 1 article reviews

vent cap cell culture flasks - by Bioz Stars, 2026-09

90/100 stars

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Cell Culture:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: Transient HeLa transfections, to assess the activity of plasmid-encoded nucleases, were also performed using 10 mg of plasmid and SuperFect reagent (QIAGEN), as per the manufacturer’s instructions, but without any subsequent selection. .. To generate the NuPro-1S cell line, HEK293F cells were seeded at 1 106 cells/mL, typically in a culture volume of 10 mL, in 125 mL vent cap cell culture flasks (Corning, UK). .. Immediately post seeding 20 mg of plasmid DNA and the Fugene6 transfection reagent (Promega, UK) was added to cells as per the manufacturer’s protocol.

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: .. Vector production by transient transfection of cells in suspension AAV vectors were produced in suspension cell culture by transient transfection using a process adapted from Zhao et al. and graphically summarized in Figure 5A.9 Suspension cells were seeded at 1 106 cells/mL in a culture volume of 30 mL in 125 mL vent cap cell culture flasks (Corning). .. The cell culture was transfected immediately postseeding using PEIpro transfection reagent (ref. no. 101000017; Polyplus Transfection, France) with helper plasmid pAdDeltaF6, payload plasmid pscAAV-GFP, and either pAAV2/5 or pAAV2/9n plasmid encoding capsid serotype 5 or and capsid serotype 9 assembly.

Transfection:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: .. Vector production by transient transfection of cells in suspension AAV vectors were produced in suspension cell culture by transient transfection using a process adapted from Zhao et al. and graphically summarized in Figure 5A.9 Suspension cells were seeded at 1 106 cells/mL in a culture volume of 30 mL in 125 mL vent cap cell culture flasks (Corning). .. The cell culture was transfected immediately postseeding using PEIpro transfection reagent (ref. no. 101000017; Polyplus Transfection, France) with helper plasmid pAdDeltaF6, payload plasmid pscAAV-GFP, and either pAAV2/5 or pAAV2/9n plasmid encoding capsid serotype 5 or and capsid serotype 9 assembly.

Suspension:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: .. Vector production by transient transfection of cells in suspension AAV vectors were produced in suspension cell culture by transient transfection using a process adapted from Zhao et al. and graphically summarized in Figure 5A.9 Suspension cells were seeded at 1 106 cells/mL in a culture volume of 30 mL in 125 mL vent cap cell culture flasks (Corning). .. The cell culture was transfected immediately postseeding using PEIpro transfection reagent (ref. no. 101000017; Polyplus Transfection, France) with helper plasmid pAdDeltaF6, payload plasmid pscAAV-GFP, and either pAAV2/5 or pAAV2/9n plasmid encoding capsid serotype 5 or and capsid serotype 9 assembly.

Bioprocessing:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: .. Vector production by transient transfection of cells in suspension AAV vectors were produced in suspension cell culture by transient transfection using a process adapted from Zhao et al. and graphically summarized in Figure 5A.9 Suspension cells were seeded at 1 106 cells/mL in a culture volume of 30 mL in 125 mL vent cap cell culture flasks (Corning). .. The cell culture was transfected immediately postseeding using PEIpro transfection reagent (ref. no. 101000017; Polyplus Transfection, France) with helper plasmid pAdDeltaF6, payload plasmid pscAAV-GFP, and either pAAV2/5 or pAAV2/9n plasmid encoding capsid serotype 5 or and capsid serotype 9 assembly.

Produced:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity
Article Snippet: .. Vector production by transient transfection of cells in suspension AAV vectors were produced in suspension cell culture by transient transfection using a process adapted from Zhao et al. and graphically summarized in Figure 5A.9 Suspension cells were seeded at 1 106 cells/mL in a culture volume of 30 mL in 125 mL vent cap cell culture flasks (Corning). .. The cell culture was transfected immediately postseeding using PEIpro transfection reagent (ref. no. 101000017; Polyplus Transfection, France) with helper plasmid pAdDeltaF6, payload plasmid pscAAV-GFP, and either pAAV2/5 or pAAV2/9n plasmid encoding capsid serotype 5 or and capsid serotype 9 assembly.

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